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Expression of Native GABA A Receptors in Xenopus Oocytes Injected With Rat Brain Synaptosomes

Sanna, Enrico ; Motzo, Costantino ; Murgia, Antonella ; Amato, Francesco ; Deserra, Tiziana ; Biggio, Giovanni

Journal of Neurochemistry, November 1996, Vol.67(5), pp.2212-2214 [Rivista Peer Reviewed]

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  • Titolo:
    Expression of Native GABA A Receptors in Xenopus Oocytes Injected With Rat Brain Synaptosomes
  • Autore: Sanna, Enrico ; Motzo, Costantino ; Murgia, Antonella ; Amato, Francesco ; Deserra, Tiziana ; Biggio, Giovanni
  • Descrizione: Oocytes from the frog were shown recently to express native nicotinic acetylcholine receptors after injection with purified electroplaque membrane vesicles. Injection of oocytes with rat cortical or nigral synaptosomes has now been shown to result in the expression of γ‐aminobutyric acid type A (GABA) receptor‐mediated Cl currents. Electrophysiological characterization of the responses of these receptors to GABA and other agents revealed that they were incorporated into the oocyte membrane and that they retained their original pharmacological properties, such as sensitivity to Cl channel blockers, benzodiazepines, and general anesthetics. These results suggest that this approach to the expression of heterologous proteins in oocytes may facilitate the study of native synaptic proteins derived from brain tissue.
  • Fa parte di: Journal of Neurochemistry, November 1996, Vol.67(5), pp.2212-2214
  • Soggetti: Oocytes ; Gaba Receptors ; Rat Brain Synaptosomes ; Heterologous Expression ; Chloride Currents ; Voltage Clamp
  • Identificativo: ISSN: 0022-3042 ; E-ISSN: 1471-4159 ; DOI: 10.1046/j.1471-4159.1996.67052212.x

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