skip to main content
Lingue:

Inhibited cell growth and protein functional changes from an editing-defective tRNA synthetase

Bacher, Jamie M ; De Crécy-Lagard, Valérie ; Schimmel, Paul R

Proceedings of the National Academy of Sciences of the United States of America, 01 February 2005, Vol.102(5), pp.1697-701 [Rivista Peer Reviewed]

Accesso online

Citazioni Citato da
  • Titolo:
    Inhibited cell growth and protein functional changes from an editing-defective tRNA synthetase
  • Autore: Bacher, Jamie M ; De Crécy-Lagard, Valérie ; Schimmel, Paul R
  • Descrizione: The genetic code is established in aminoacylation reactions catalyzed by aminoacyl-tRNA synthetases. Many aminoacyl-tRNA synthetases require an additional domain for editing, to correct errors made by the catalytic domain. A nonfunctional editing domain results in an ambiguous genetic code, where a single codon is not translated as a specific amino acid but rather as a statistical distribution of amino acids. Here, wide-ranging consequences of genetic code ambiguity in Escherichia coli were investigated with an editing-defective isoleucyl-tRNA synthetase. Ambiguity retarded cell growth at most temperatures in rich and minimal media. These growth rate differences were seen regardless of the carbon source. Inclusion of an amino acid analogue that is misactivated (and not cleared) diminished growth rate by up to 100-fold relative to an isogenic strain with normal editing function. Experiments with target-specific antibiotics for ribosomes, DNA replication, and cell wall biosynthesis, in conjunction...
  • Fa parte di: Proceedings of the National Academy of Sciences of the United States of America, 01 February 2005, Vol.102(5), pp.1697-701
  • Soggetti: Amino Acyl-Trna Synthetases -- Metabolism ; Cell Division -- Physiology ; Escherichia Coli -- Genetics ; RNA Editing -- Genetics
  • Lingua: Inglese
  • Identificativo: ISSN: 0027-8424 ; PMID: 15647356 Version:1

Ricerca in corso nelle risorse remote ...