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Method for analyzing and synthesizing functional polypeptide sequences for assembly into enhanced or multi-functional proteins

Jasin; Maria ; Schimmel; Paul R

28 August 1990

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  • Titolo:
    Method for analyzing and synthesizing functional polypeptide sequences for assembly into enhanced or multi-functional proteins
  • Autore: Jasin; Maria ; Schimmel; Paul R
  • Anno: 28 August 1990
  • Descrizione: Disclosed is a method for identifying and isolating gene sequences coding for polypeptide sequences which result in specific functional activities or phenotypes, which may then be synthesized and assembled to form new proteins. The assembled protein may contain only amino acid sequences essential for specific functions, have a new quaternary structure resulting from addition of an amino acid sequence with oligomeric activity, or exhibit multiple activities. Also disclosed is a method for identifying, analyzing and synthesizing amino acid sequences with oligomeric activity which may be used to enhance or add a new activity to a protein. In one embodiment, the piece with oligomeric activity is fused onto a polypeptide with one or more additional activities. In another embodiment, this polypeptide sequence is hybridized with a second polypeptide consisting only of the sequence with oligomeric activity. In a variation of this second method, the first polypeptide may naturally contain a sequence with oligomeric activity and the second polypeptide sequence may be synthesized to complement the naturally occurring sequence. The protein-to-protein interactions serve to stabilize or enhance the activities on the first polypeptide sequences. These methods provide means for enhancing activity in sequences that occur naturally or in sequences which are synthesized from a variety of sources. The coupling piece may be spatially remote from the active or functional domain and still be effective in producing a protein with enhanced or restored or stabilized activity.
  • Soggetti: Chemistry ; Metallurgy ; Biochemistry ; Beer ; Spirits ; Wine ; Vinegar ; Microbiology ; Enzymology ; Mutation OR Genetic Engineering ; Microorganisms OR Enzymes ; Compositions Thereof ; Propagating, Preserving OR Maintaining Microorganisms ; Mutation OR Genetic Engineering ; Culture Media ; Medicine ; Sciences ; Chemistry ; Chemistry
  • Lingua: Inglese
  • Fonte: Espacenet (European Patent Office)

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